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The Iso-Seq express 2.0 kit provides reagents and oligos for generating full-length cDNA. This kit includes 12 unique barcoded cDNA primers for multiplexing and is compatible with the Kinnex full-length RNA kit. All components are conveniently packaged in a single box, supporting 24 reactions per kit.
Contains reagents and oligos for generating full-length cDNA.
Includes 12 unique barcoded cDNA primers for multiplexing.
Compatible with the Kinnex full-length RNA kit.
Supports 24 reactions per kit.
Compatible with the Revio system, Vega system, and Sequel II and IIe systems.
Store frozen between -15°C to -25°C.
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Leupeptin hemisulfate (Ac-Leu-Leu-argininal) is a reversible inhibitor of serine and cysteine endopeptidases Leupeptin hemisulfate is capable of inhibiting plasmin trypsin papain kallikrein calpain and cathepsin B Leupeptin is usually used in a concentration range of 10 to 100 M Leupeptin is widely used in many protease inhibitor cocktails due to its potency low toxicity and range of inhibition Leupeptin is also used in a quantitative assay to measure macroautophagic flux in mammals and is being investigated for its use in treating malaria
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The EasySep™ Human Monocyte Isolation Kit is designed to isolate CD14+CD16- monocytes from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells and platelets with antibody complexes and magnetic particles. The magnetically labelled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This product can be used in place of the EasySep™ Human Monocyte Enrichment Kit (Catalog #19059) for even faster cell isolations with reduced platelet contamination.
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The EasySep™ Mouse NK Cell Isolation Kit targets non-NK cells by labeling unwanted cells with biotinylated antibodies and EasySep™ Streptavidin RapidSpheres™, and separates cells without columns using an EasySep™ magnet. Desired cells are simply poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture or cell-based assays.This product replaces the EasySep™ Mouse NK Cell Enrichment Kit (Catalog #19755) for even faster cell isolations.
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The EasySep™ Mouse CD117 (cKIT) Positive Selection Kit is designed to isolate CD117+ cells from single-cell suspensions of bone marrow by positive selection. The cocktail contains a combination of PE-labeled antibodies that is directed against CD117, which are then recognized by antibody complexes that are directed against PE and dextran. Labeled cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
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Mitochondria/cytosol fractionation kit provides a unique formulation of reagents for the effective isolation of highly enriched mitochondrial fractions from the cytosolic fraction of mammalian cells, including both apoptotic and non-apoptotic cells. - Cited in over 110 publications - Isolates mitochondrial and cytosolic fractions for studying apoptotic pathways and signal transduction - Simple and easy procedure for fractionation - Suitable for detecting translocation of factors between fractions via Western blotting (WB), ELISA, or other assays
Mitochondria/cytosol fractionation kit provides a unique formulation of reagents for the effective isolation of highly enriched mitochondrial fractions from the cytosolic fraction of mammalian cells, including both apoptotic and non-apoptotic cells. - Cited in over 110 publications - Isolates mitochondrial and cytosolic fractions for studying apoptotic pathways and signal transduction - Simple and easy procedure for fractionation - Suitable for detecting translocation of factors between fractions via Western blotting (WB), ELISA, or other assays
Plasma membrane protein extraction kit provides optimized buffers and reagents for the effective extraction of plasma membrane proteins from mammalian tissues and cells. The kit offers the option to either extract total cellular membrane proteins or specifically purify plasma membrane proteins. - Cited in over 200 publications - High yield and purity – consistent yield with high purity (over 90%) - Suitable for western blotting, 2-D gels, enzyme analyses, and more